Modulation of methotrexate resistance by genistein in murine leukemia L1210 cells.

  title={Modulation of methotrexate resistance by genistein in murine leukemia L1210 cells.},
  author={Yongzhi Xuan and Miles P. Hacker and Thomas R. Tritton and Alok Bhushan},
  journal={Oncology reports},
  volume={5 2},
We have previously shown that methotrexate (MTX) transport is impaired in murine L1210 cells selected for cisplatin (DDP) resistance (L1210/DDP) and that the decreased MTX uptake may be due to an altered 66 kDa membrane protein. We have further hypothesized that tyrosine phosphorylation is necessary for the function of this protein. To determine the importance of tyrosine phosphorylation we studied the effect of genistein, a tyrosine kinase inhibitor, on methotrexate sensitivity, uptake and… Expand
Collateral methotrexate resistance in cisplatin-selected murine leukemia cells.
  • A. Bhushan, M. Hacker, T. Tritton
  • Biology, Medicine
  • Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
  • 1999
Resistance to anticancer drugs is a major cause of failure of many therapeutic protocols. A variety of mechanisms have been proposed to explain this phenomenon. The exact mechanism depends upon theExpand
Identification and characterization of a 66–68-kDa protein as a methotrexate-binding protein in murine leukemia L1210 cells
The HSC70 protein, cloned from the L1210 murine leukemia cells, was expressed and purified from E. coli cells using a polyhistidine-tag purification system and it also showed the binding properties with MTX. Expand
Tyrosine Kinase Inhibitors in Preclinical Development
This article is intended to review those tyrosine kinase inhibitors that are currently in preclinical development, for which there are data to support consideration for their use in chemoprevention or cancer treatment. Expand
Methotrexate combination effects with genistein and daidzein on MDA-MB-231 breast cancer cell viability
Treatment of MDA-MB-231 breast cancer cells with methotrexate and genistein or daidzein produces interactions consistent with antagonism but the effects are predicted to diminish with rising metotrexate to isoflavone ratio. Expand
Pharmacological activities of Genistein, an isoflavone from soy (Glycine max): part I--anti-cancer activity.
The evidences reviewed here represent the beneficial effects of most potential and promising isoflavone, Genistein in various types of cancers. Expand