Modulation of intramolecular interactions in superhelical DNA by curved sequences: a Monte Carlo simulation study.

Abstract

A Monte Carlo model for the generation of superhelical DNA structures at thermodynamic equilibrium (Klenin et al., 1991; Vologodskii et al., 1992) was modified to account for the presence of local curvature. Equilibrium ensembles of a 2700-bp DNA chain at linking number difference delta Lk = -15 were generated, with one or two permanent bends up to 120 degrees inserted at different positions. The computed structures were then analyzed with respect to the number and positions of the end loops of the interwound superhelix, and the intramolecular interaction probability of different segments of the DNA. We find that the superhelix structure is strongly organized by permanent bends. A DNA segment with a 30 degrees bend already has a significantly higher probability of being at the apex of a superhelix than the control, and for a 120 degrees bend the majority of DNAs have one end loop at the position of the bend. The entropy change due to the localization of a 120 permanent bend in the end loop is estimated to be -17 kJ mol-1 K-1. When two bends are inserted, the conformation of the superhelix is found to be strongly dependent on their relative positions: the straight interwound form dominates when the two bends are separated by 50% of the total DNA length, whereas the majority of the superhelices are in a branched conformation when the bends are separated by 33%. DNA segments in the vicinity of the permanent bend are strongly oriented with respect to each other.

Statistics

050100150'99'01'03'05'07'09'11'13'15'17
Citations per Year

232 Citations

Semantic Scholar estimates that this publication has 232 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Klenin1995ModulationOI, title={Modulation of intramolecular interactions in superhelical DNA by curved sequences: a Monte Carlo simulation study.}, author={Konstantin V. Klenin and Maxim D. Frank-Kamenetskii and J. Langowski}, journal={Biophysical journal}, year={1995}, volume={68 1}, pages={81-8} }