Modulation of human neutrophil LTA hydrolase activity by phorbol myristate acetate.

@article{McColl1987ModulationOH,
  title={Modulation of human neutrophil LTA hydrolase activity by phorbol myristate acetate.},
  author={Shaun R McColl and Nigel Hurst and W. Henry Betts and Leslie G. Cleland},
  journal={Biochemical and biophysical research communications},
  year={1987},
  volume={147 2},
  pages={
          622-6
        }
}
The phorbol ester, phorbol 12-myristate 13-acetate enhanced leukotriene B4 production stimulated by formyl-methionyl-leucyl-phenylalanine and arachidonic acid and reduced the production of the all-trans isomers of LTB4 by human neutrophils. Production of 5-hydroxyeicosatetraenoic acid was unaffected. These observations are consistent with a stimulatory effect of phorbol ester on LTA hydrolase, the enzyme which catalyses the conversion of LTA4 to LTB4. We demonstrate that a protein of the same… CONTINUE READING