Modulation of fluoropyrimidine metabolism in L1210 cells by L-alanosine.

  title={Modulation of fluoropyrimidine metabolism in L1210 cells by L-alanosine.},
  author={Robert Heimer and D Goldberg and Edwin C. Cadman},
  journal={Biochemical pharmacology},
  volume={32 2},
L-Alanosine, an analogue of aspartic acid which inhibits the conversion of inosine monophosphate to adenosine monophosphate (AMP), was evaluated in L1210 cells as a modulator of 5-fluorouracil (FUra) and 5-fluorouridine (FUrd) metabolism. L-Alanosine resulted in increased intra-cellular levels of 5-phosphoribosyl-1-pyrophosphate (PRPP), enhanced FUra metabolism to ribonucleotide derivatives, and resulted in more FUra residues incorporated into RNA. Sequential addition of L-alanosine and FUra… Expand
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The resistance of a Novikoff hepatoma cell line (N1-S1/FdUrd) to 5-fluorouracil (FUra) was reversed by the inclusion of inosine in the culture medium and there was a marked increase in the uptake of [14C]FUra and conversion to nucleotides with a corresponding increased in the incorporation into RNA. Expand
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Alanosine toxicity in Novikoff rat hepatoma cells due to inhibition of the conversion of inosine monophosphate to adenosine monophosphate.
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The inhibition of ribosomal RNA synthesis and maturation in Novikoff hepatoma cells by 5-fluorouridine.
Results suggest that some minimum degree of analog substitution is necessary to inhibit the maturation process of ribosomal RNA (rRNA) maturation in Novikoff hepatoma cells. Expand
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