Modulation of endogenous indole-3-acetic acid biosynthesis in bacteroids within Medicago sativa nodules.

@article{Bianco2014ModulationOE,
  title={Modulation of endogenous indole-3-acetic acid biosynthesis in bacteroids within Medicago sativa nodules.},
  author={Carmen Bianco and Beatrice Senatore and Salvatore Arbucci and Giuseppe Pieraccini and Roberto Defez},
  journal={Applied and environmental microbiology},
  year={2014},
  volume={80 14},
  pages={4286-93}
}
To evaluate the dose-response effects of endogenous indole-3-acetic acid (IAA) on Medicago plant growth and dry weight production, we increased the synthesis of IAA in both free-living and symbiosis-stage rhizobial bacteroids during Rhizobium-legume symbiosis. For this purpose, site-directed mutagenesis was applied to modify an 85-bp promoter sequence, driving the expression of iaaM and tms2 genes for IAA biosynthesis. A positive correlation was found between the higher expression of IAA… CONTINUE READING
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