Modulation of antibody galactosylation through feeding of uridine, manganese chloride, and galactose.

@article{Gramer2011ModulationOA,
  title={Modulation of antibody galactosylation through feeding of uridine, manganese chloride, and galactose.},
  author={Michael J. Gramer and Jackie J Eckblad and Ruth Donahue and Joseph D Brown and Carrie Shultz and Kent Vickerman and Patrick Priem and Ewald T J van den Bremer and Jolanda Gerritsen and Patrick H.C. van Berkel},
  journal={Biotechnology and bioengineering},
  year={2011},
  volume={108 7},
  pages={1591-602}
}
Through process transfer and optimization for increased antibody production to 3 g/L for a GS-CHO cell line, an undesirable drop in antibody Fc galactosylation was observed. Uridine (U), manganese chloride (M), and galactose (G), constituents involved in the intracellular galactosylation process, were evaluated in 2-L bioreactors for their potential to specifically increase antibody galactosylation. These components were placed in the feed medium at proportionally increasing concentrations from… CONTINUE READING

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