BACKGROUND Nanotechnology is rapidly emerging in biomedical applications, including cancer therapy. Here, a mixture of ultra dispersed nanodiamond and nanoplatinum was coated onto fabrics in the form of a cloth (DPV576-C). The role of DPV576-C in modulating T lymphocytes of aged mice was examined. MATERIALS AND METHODS C57BL/6 mice were treated with DPV576-C as a lining in a mouse house for 1 month. Splenic cells were analyzed for CD4(+) and CD8(+) T-cells and NK activity using flow cytometry. RESULTS DPV576-C-treated aged mice showed an: (1) increase in the percentages of CD4(+) and CD8(+) T-cells and their activation markers, CD25 and CD69, over untreated aged mice; (2) enhancement of NK activity; and (3) absence of adverse side effects as determined histopathologically. CONCLUSION The enhancement of lymphocytes by DPV576-C may be useful for patients suffering from immune dysfunction.