Modulation of adenovirus-mediated gene transfer by nitric oxide.

  title={Modulation of adenovirus-mediated gene transfer by nitric oxide.},
  author={Imad Y. Haddad and Eric Sorscher and Robert I. Garver and Jau-Shyong Hong and Edith E. Tzeng and Sadis Matalon},
  journal={American journal of respiratory cell and molecular biology},
  volume={16 5},
We assessed the role of .NO in recombinant adenovirus-mediated gene transfer both in vitro and in vivo. NIH3T3 fibroblasts, stably transfected with the human inducible nitric oxide synthase, but lacking tetrahydrobiopterin (NIH3T3/iNOS [inducibile nitric oxide synthase]), were infected with replication-deficient adenovirus (E1-deleted), containing either the luciferase or the Lac Z reporter genes (AdCMV-Luc and AdCMV-Lac Z; 1-10 plaque forming units [pfu]/cell). Incubation of infected cells… CONTINUE READING