Modulation of K+ currents in monocytes by VCAM-1 and E-selectin on activated human endothelium.

@article{ColdenStanfield1998ModulationOK,
  title={Modulation of K+ currents in monocytes by VCAM-1 and E-selectin on activated human endothelium.},
  author={Margaret Colden-Stanfield and Elaine K. Gallin},
  journal={American journal of physiology. Cell physiology},
  year={1998},
  volume={275 1},
  pages={C267-C277}
}
Resting membrane potential (RMP) and whole cell currents were recorded in human THP-1 monocytes adherent to polystyrene, unstimulated human umbilical vein endothelial cells (HUVECs), lipopolysaccharide (LPS)-treated HUVECs, immobilized E-selectin, or vascular cell adhesion molecule 1 (VCAM-1) using the patch-clamp technique. RMP after 5 h on polystyrene was -24.3 ± 1.7 mV ( n = 42) with delayed rectifier K+( I dr) and Cl- currents ( I Cl) present in >75% of the cells. Inwardly rectifying K… CONTINUE READING