Modulation of GABA receptor binding by Ca2+.

  title={Modulation of GABA receptor binding by Ca2+.},
  author={Maria Giuseppa Corda and Alessandro Guidotti},
  journal={Journal of neurochemistry},
  volume={41 1},
In frozen-thawed repeatedly washed rat cortical synaptic membranes, Ca2+ (1-5 mM) decreased the binding of [3H]muscimol whereas it increased the binding of [3H]gamma-aminobutyric acid (GABA). However, the binding of [3H]GABA was decreased by the same extent as the binding of [3H]muscimol when the membranes were incubated with baclofen (a selective ligand for the GABAB binding site) and Ca2+. Scatchard analysis of [3H]muscimol binding revealed that Ca2+ reduced the density of GABA binding sites… CONTINUE READING