Modulation of Aspergillus awamori thaumatin secretion by modification of bipA gene expression.

@article{Lombraa2004ModulationOA,
  title={Modulation of Aspergillus awamori thaumatin secretion by modification of bipA gene expression.},
  author={Marta Lombra{\~n}a and Francisco J Moralejo and Rosa Maria Ferreiro Pinto and Juan F. Martin},
  journal={Applied and environmental microbiology},
  year={2004},
  volume={70 9},
  pages={5145-52}
}
Two different strains, Aspergillus awamori TGDTh-4 and A. awamori TGP-3 overexpressing a synthetic gene encoding the plant sweet protein thaumatin, showed an unfolded protein response. To facilitate protein secretion, the chaperone BiPA gene was expressed in A. awamori under control of the strong constitutive promoter of the gpdA gene. A good correlation was observed between the level of the bipA transcript in different strains and the amount of thaumatin secreted. Thaumatin secretion was… CONTINUE READING