Modulation of Antigenic Phenotype in Cultured Human Osteoblast-like Cells by FGFb, TGFb1, PDGF-BB, IL-2, IL-1b, LPS and IFNc

Abstract

Background/Aims Recent reports demonstrated that osteoblast-like cells can also exert activities directly associated with the immune system (cytokine synthesis, antigen presentation, phagocytosis and stimulation of T lymphocytes). The present study aimed to analyze the effect of Transforming growth factorb1 (TGFb1), Fibroblast growth factor basic (FGFb), Platelet-derived growth factor-BB (PDGF-BB), Interleukin-1b (IL-1b), Interleukin-2 (IL-2), Lipopolysaccharide (LPS) and Interferon-c (IFNc) on the expression on osteoblast-like cells of antigens involved in antigen presentation. Methods Flow cytometry was used to investigate whether the growth factors FGFb, TGFb1, PDGF-BB, IL-2, IL-1b, LPS and IFNc modulate the expression on cultured human osteoblast-like cells of different antigens involved in antigen-presentation and T cell activation. Results TGFb1 treatment significantly reduced the expression of CD54 and CD86. IL1b treatment significantly enhanced the expression of CD54, CD86 and HLA-DR. LPS and IFNc treatments produced a major increase in CD54, CD80, CD86 and HLA-DR expression. Expression of these antigen-presenting molecules was not significantly modified by FGFb, PDGF-BB or IL-2 treatment. E. Pérez Æ O. Garcı́a-Martı́nez Æ C. Ruiz Department of Nursing, Physiology Section, University of Granada, E-18012 Granada, Spain M. Arroyo-Morales Department of Physiotherapy, University of Granada, E-18012 Granada, Spain C. Reyes-Botella Department of Stomatology, University of Granada, E-18012 Granada, Spain C. Ruiz (&) Escuela Universitaria de Ciencias de la Salud, University of Granada, Avda. Madrid s/n., 18071 Granada, Spain e-mail: crr@ugr.es Biosci Rep (2006) 26:281–289 DOI 10.1007/s10540-006-9022-z 123 ORI GI N A L P A PE R Modulation of Antigenic Phenotype in Cultured Human Osteoblast-like Cells by FGFb, TGFb1, PDGF-BB, IL-2, IL-1b, LPS and IFNc E. Pérez Æ O. Garcı́a-Martı́nez Æ M. Arroyo-Morales Æ C. Reyes-Botella Æ C. Ruiz Published online: 22 September 2006 Springer Science+Business Media, Inc. 2006

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@inproceedings{Prez2006ModulationOA, title={Modulation of Antigenic Phenotype in Cultured Human Osteoblast-like Cells by FGFb, TGFb1, PDGF-BB, IL-2, IL-1b, LPS and IFNc}, author={Ever P{\'e}rez}, year={2006} }