GPER mediates estrogen-induced signaling and proliferation in human breast epithelial cells and normal and malignant breast.
Dedication This work is dedicated to my grandpa George. Your Texas Tornado loves you with all her heart. v Acknowledgements I would like to first and foremost offer my gratitude to my advisor Peter Thomas, for his vast knowledge on membrane steroid receptors and time and guidance spent improving my writing and communication skills especially. Thank you to Dr. and Dr. Md Saydur Rahman for their feedback on this project and thesis. To Susan Lawson, for her teachings and assistance with fish care and samplings, without which this project would not have been possible. To my labmate Aubrey Converse, for her expertise on all things ZIP9, patient willingness to lend her time to my experiments, and unconditional friendship. To my labmate Amanda Fitzgerald, for lending me her time during samplings and her calm demeanor. To Jing Dong, for her patient help and warm smile. To Dr. Yefei Pang, for his teachings and special attention during my time as an undergraduate in this lab. To Dr. Yosi Aizen, for his teachings and sense of humor. To Dr. Wenxian Tan, for his advice and contagious zest for life, and Pi, for our time as roommates. To my cohort, my confidant Tracy Harvey, my dive buddy Sara Wilson, roommate Stephanie Smith, academic advisor and comedienne Jordann Young, and the brilliant Kaijun Lu, for solidarity throughout the past three years together, from our very first introduction in the basement of the BIO building in Austin. To my parents, Doug and Janet Ondricek, who taught me how to fish (albeit, without a hook) and so much more. And finally, thank you to all the wonderful people I've met during my time in Port Aransas and at the Marine Abstract Modulation by hypoxia of membrane steroid receptor expression and functions in ovaries of Atlantic croaker (Micropogonias undulatus) Hypoxia is an endocrine disruptor, altering estrogen, testosterone, and progestin hormone levels and stunting gonadal growth in Atlantic croaker. Steroids act through specific hormone receptors to alter reproductive functions, and the hormonal response is dependent on the concentrations of these receptors. However, information is currently lacking on the effects of hypoxia on expression and functions of membrane receptors mediating rapid, non-genomic steroid actions such as final oocyte maturation and apoptosis. Atlantic croakers were exposed to normoxia (7.0 mg DO/L) or hypoxia (1.7 mg DO/L) for 6 weeks during their period of gonadal recrudescence (October-December). Relative gene expression was quantified …