Modulating the Nucleophile of a Glycoside Hydrolase through Site-Specific Incorporation of Fluoroglutamic Acids.

@article{Koetzler2018ModulatingTN,
  title={Modulating the Nucleophile of a Glycoside Hydrolase through Site-Specific Incorporation of Fluoroglutamic Acids.},
  author={Miriam P. Koetzler and K. Robinson and Hong-ming Chen and M. Okon and L. McIntosh and S. Withers},
  journal={Journal of the American Chemical Society},
  year={2018},
  volume={140 26},
  pages={
          8268-8276
        }
}
Understanding the detailed mechanisms of enzyme-catalyzed hydrolysis of the glycosidic bond is fundamentally important, not only to the design of tailored cost-efficient, stable and specific catalysts but also to the development of specific glycosidase inhibitors as therapeutics. Retaining glycosidases employ two key carboxylic acid residues, typically glutamic acids, in a double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. One Glu functions as a nucleophile while… Expand
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