Modular bioreactor for primary human hepatocyte culture: Medium flow stimulates expression and activity of detoxification genes

@inproceedings{Vinci2011ModularBF,
  title={Modular bioreactor for primary human hepatocyte culture: Medium flow stimulates expression and activity of detoxification genes},
  author={B. Vinci and C{\'e}dric Duret and Sylvie Klieber and Sabine Gerbal-chaloin and Antonio Sa-Cunha and Sylvain Laporte and Bertrand Suc and Patrick Maurel and Arti Ahluwalia and Martine Daujat-Chavanieu},
  booktitle={Biotechnology journal},
  year={2011}
}
Down-regulation of detoxification genes, notably cytochrome P450 (CYPs), in primary hepatocyte cultures is a long-standing and major concern. We evaluated the influence of medium flow in this model. Hepatocytes isolated from 12 different liver donors were cultured either in a multichamber modular bioreactor (MCmB, flow rate 250-500 μL/min) or under standard/static conditions, and the expression of 32 genes, enzyme activities and biological parameters were measured 7-21 days later. mRNA… CONTINUE READING

From This Paper

Figures, tables, results, connections, and topics extracted from this paper.
23 Extracted Citations
0 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 23 extracted citations

Similar Papers

Loading similar papers…