Modifying the genetic code: Converting nonsense codons into sense codons by targeted pseudouridylation

@inproceedings{Karijolich2011ModifyingTG,
  title={Modifying the genetic code: Converting nonsense codons into sense codons by targeted pseudouridylation},
  author={John Karijolich and Yi-Tao Yu},
  booktitle={Nature},
  year={2011}
}
All three translation termination codons, or nonsense codons, contain a uridine residue at the first position of the codon. Here, we demonstrate that pseudouridylation (conversion of uridine into pseudouridine (Ψ), ref. 4) of nonsense codons suppresses translation termination both in vitro and in vivo. In vivo targeting of nonsense codons is accomplished by the expression of an H/ACA RNA capable of directing the isomerization of uridine to Ψ within the nonsense codon. Thus, targeted… CONTINUE READING
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