Modifying the beta,gamma leaving-group bridging oxygen alters nucleotide incorporation efficiency, fidelity, and the catalytic mechanism of DNA polymerase beta.

@article{Sucato2007ModifyingTB,
  title={Modifying the beta,gamma leaving-group bridging oxygen alters nucleotide incorporation efficiency, fidelity, and the catalytic mechanism of DNA polymerase beta.},
  author={Christopher A Sucato and Thomas G Upton and Boris A. Kashemirov and Vinod K. Batra and V{\'a}clav Mart{\'i}nek and Yun Xiang and William A. Beard and Lars C Pedersen and Samuel H. Wilson and Charles E McKenna and Jan Flori{\'a}n and Arieh Warshel and Myron F. Goodman},
  journal={Biochemistry},
  year={2007},
  volume={46 2},
  pages={461-71}
}
DNA polymerase catalysis and fidelity studies typically compare incorporation of "right" versus "wrong" nucleotide bases where the leaving group is pyrophosphate. Here we use dGTP analogues replacing the beta,gamma-bridging O with CH2, CHF, CF2, or CCl2 to explore leaving-group effects on the nucleotidyl transfer mechanism and fidelity of DNA polymerase… CONTINUE READING