Modifying fatty acid profiles through a new cytokinin-based plastid transformation system.

@article{Dunne2014ModifyingFA,
  title={Modifying fatty acid profiles through a new cytokinin-based plastid transformation system.},
  author={Aisling Dunne and Jodi Maple-Gr\odem and Daniela Gargano and Richard P. Haslam and Johnathan A Napier and Nam-Hai Chua and Rosalind L Russell and Simon G M\oller},
  journal={The Plant journal : for cell and molecular biology},
  year={2014},
  volume={80 6},
  pages={1131-8}
}
The widespread use of herbicides and antibiotics for selection of transgenic plants has not been very successful with regard to commercialization and public acceptance. Hence, alternative selection systems are required. In this study, we describe the use of ipt, the bacterial gene encoding the enzyme isopentenyl transferase from Agrobacterium tumefaciens, as a positive selectable marker for plastid transformation. A comparison between the traditional spectinomycin-based aadA selection system… CONTINUE READING
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