Modified siRNA effectively silence inducible immunoproteasome subunits in NSO cells.

@article{Gvozdeva2016ModifiedSE,
  title={Modified siRNA effectively silence inducible immunoproteasome subunits in NSO cells.},
  author={Olga V Gvozdeva and Alexey Belogurov and Ekaterina S Kuzina and Alexandre G. Gabibov and Mariya I. Meschaninova and Alya G. Ven'yaminova and Marina A. Zenkova and Valentin V. Vlassov and Elena L. Chernolovskaya},
  journal={Biochimie},
  year={2016},
  volume={125},
  pages={75-82}
}
The pathogenesis of autoimmune and neurodegenerative diseases involves overexpression of inducible subunits of the immunoproteasome. However, the clinical application of inhibitors to inducible subunits of the immunoproteasome has been limited due to systemic toxicity. Here, we designed siRNAs that efficiently silence LMP2, LMP7 and MECL-1 gene expression. Inducible subunits of the immunoproteasome are complex siRNA targets because they have a long half-life; therefore, we introduced 2'-O… CONTINUE READING