Modified paper chromatographic method for assay of myocardial depressant factor.

A simplified paper chromatographic technique for assaying myocardial depressant factor (MDF) is presented. In addition to a serine standard, deproteinized plasma samples from rabbits subjected to hemorrhagic shock were applied to paper and chromatographed. The spot in the chromatograms occurring at a migration distance of 1.6 to 1.8 times that of the… (More)