Modified nucleosides and bizarre 5′-termini in mouse myeloma mRNA

@article{Adams1975ModifiedNA,
  title={Modified nucleosides and bizarre 5′-termini in mouse myeloma mRNA},
  author={Jerry M. Adams and Suzanne Cory},
  journal={Nature},
  year={1975},
  volume={255},
  pages={28-33}
}
Messenger RNAs from mouse myeloma cells contain N6-methyl adenosine and novel 5′ termini having 7-methylguanosine in a 5′, 5′triphosphate linkage with ribose-methylated nucleosides. Ten common 5′-terminal sequences of the forms m7G5′ppp5′NmpNp and m7G5′ppp5′NmpNmpNp are reported. Structures like this may be a general feature of mRN A in eukaryotes. 

N6, O2′-dimethyladenosine a novel methylated ribonucleoside next to the 5′ terminal of animal cell and virus mRNAs

A methylated nucleoside that chromatographed slightly ahead of 2′-O-methyladenosine (Am) is identified and it is found that it is common to mRNAs of human and mouse cells as well as at least one virus.

Modified 5′-termini in small nuclear RNAs of mouse myeloma cells

Nuclei of MPC 11 mouse myeloma cells contain several species of small RNAs related to those found in other mammalian cells, and the 5′-termini of 32P-labelled U1 and U2 RNAs have been investigated by a fingerprinting technique involving digestion with T2-ribonuclease.

Blocked, methylated 5′-terminal sequence in avian sarcoma virus RNA

A blocked, methylated 5′-terminal structure—m7GpppGmpCp—in the genome RNA of avian sarcoma virus (ASV) is found.

THE SYNTHESIS OF α,γ-DINUCLEOSIDE TRIPHOSPHATES. THE CONFRONTED NUCLEOTIDE STRUCTURE FOUND AT THE 5′-TERMINUS OF EUKARYOTE MESSENGER RIBONUCLEIC ACID

α,γ-Dinucleoside triphosphates showing the recently discovered modified structure at the 5′-terminus of eukaryote mRNA were synthesized by use of a new method employing di-n-butylphosphinothioyl

Evidence for role of m7G5′-phosphate group in recognition of eukaryotic mRNA by initiation factor IF-M3

Evidence is provided that the role of 7-methylguanosine in the mechanism for initiation of eukaryotic mRNA translation may be related to specific recognition of mRNA by initiation factor IF-M3.
...

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  • Y. Furuichi
  • Biology, Chemistry
    Nucleic acids research
  • 1974
The dependence of the viral transcription on the presence of SAM and the methylation of terminal nucleotide suggests that the transcription of CPV is a "methylation-coupled" reaction.