Modified bases in RNA reduce secondary structure and enhance hybridization.

@article{Gamper2004ModifiedBI,
  title={Modified bases in RNA reduce secondary structure and enhance hybridization.},
  author={Howard B. Gamper and Alan Gewirtz and Jillian Edwards and Yangshao Hou},
  journal={Biochemistry},
  year={2004},
  volume={43 31},
  pages={
          10224-36
        }
}
Secondary structure in RNA targets is a significant barrier to short DNA probes. However, when such targets are the end product of an in vitro amplification scheme, it is possible to carry out transcription in the presence of nucleoside triphosphate analogues that reduce secondary structure of the RNA without impairing subsequent hybridization. Here we show that nucleoside triphosphates of 2-aminoadenine (nA) and 2-thiouracil (sU) are taken up by T7 RNA polymerase and that the resulting RNA… CONTINUE READING
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