Modified bacteriophage lambda promoter vectors for overproduction of proteins in Escherichia coli.

@article{Elvin1990ModifiedBL,
  title={Modified bacteriophage lambda promoter vectors for overproduction of proteins in Escherichia coli.},
  author={Chris Elvin and Paul R Thompson and M E Argall and Philip Hendry and N P Stamford and Penelope E. Lilley and Nicholas E Dixon},
  journal={Gene},
  year={1990},
  volume={87 1},
  pages={123-6}
}
A new series of expression vectors that direct high-level overproduction of gene products in Escherichia coli is described. All contain strong bacteriophage lambda promoters, PR and PL, arranged in tandem so that both promote transcription into genes inserted into or between unique restriction sites. The vectors also direct expression of the lambda cI857 gene (from its natural promoter, PM), which enables their use in any E. coli host strain to effect controlled expression by shifting the… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 40 extracted citations

Promoter and Terminator Discovery and Engineering.

Advances in biochemical engineering/biotechnology • 2018
View 1 Excerpt

Synthetic biology: an emerging engineering discipline.

Annual review of biomedical engineering • 2012
View 1 Excerpt

Similar Papers

Loading similar papers…