Modifications of two methods for the assay of amylase.

@article{Somogyi1960ModificationsOT,
  title={Modifications of two methods for the assay of amylase.},
  author={Michael Somogyi},
  journal={Clinical chemistry},
  year={1960},
  volume={6},
  pages={
          23-35
        }
}
  • M. Somogyi
  • Published 1960
  • Chemistry, Medicine
  • Clinical chemistry
N 1938 I DESCRIBED TWO METHODS for the assay of amylase in biologic material (1) and subsequently published excerpts of studies (2, 3) dealing with the kinetics of the saccharogenic action of the amylase that served as the basis for my first analytic procedure. The second method, which is based on the amyloclastic action, yields results that run quantitatively parallel with those obtained by the first method; its simplicity and rapidity recommend it as a convenient tool for clinical diagnostic… Expand

Tables and Topics from this paper

An appraisal of methods for serum amylase determination
Abstract Of all the methods used to assay amylase activity, the saccharogenic techniques appear to possess the greatest intrinsic validity. Amyloclastic procedures entailing use of the starch-iodineExpand
Laboratory evaluation of a chromogenic amylase method.
  • F. Dalal, S. Winsten
  • Chemistry, Medicine
  • Clinica chimica acta; international journal of clinical chemistry
  • 1971
TLDR
Minor modifications of the commercial method seem to result in a sensitive and reliable procedure, which compares favorably with the amyloclastic assay, and the substrate appears to react within a broad pH range. Expand
The electrophoretic nature of human amylase and the effect of protein on the starch-iodine reaction.
  • P. Wilding
  • Chemistry, Medicine
  • Clinica chimica acta; international journal of clinical chemistry
  • 1965
TLDR
The results obtained indicate that on electrophoresis the amylase of human serum migrates only with the γ-globulin fraction, and that previous results obtained by some workers using amyloclastic methods for investigating this subject are invalid. Expand
The coulometric determination of amylase in serum.
TLDR
The accuracy and precision of the coulometric and a colorimetric method are comparable, but both are limited by difficulties in the steps preceding measurement rather than in the measurement step itself. Expand
Preparation and application of Procion Yellow starch for amylase assay.
  • D. Jung
  • Chemistry, Medicine
  • Clinica chimica acta; international journal of clinical chemistry
  • 1980
TLDR
A new starch derivative for the determination of amylase activity has been synthesized by coupling Procion Yellow dye with starch, which is intensely yellow and is easily suspended in water, in neutral buffer solution, and also in acidic solution. Expand
Biochemistry of human alpha amylase isoenzymes.
TLDR
The purpose of this article is to review recent literature on the isoenzymes of alpha amylase to bring together findings that may be of interest to clinical laboratory physicians and scientists. Expand
A New Saccharogeni . cMicromethodfor Measurement of AmylaseActivity in BiologicalFluids
To determine serum amylase activity we have quantitatively measured the glucose and maltose hydrolyzed from soluble starch by colorimetrically measuring the reduction of colorlessExpand
New AmylaseSubstrateand AssayProcedure
This report describes a new procedure for serum amylase assay. A novel substrate, dyed amylopectin, is used that combines the advantages of saccharogenic and amyloclastic methods. Serum amylaseExpand
Determination of Serumand UrineAmylase with Useof ProcionBrilliantRed M-2BSAmylopectin
We describe a sensitive, precise assay of serum or urine amylase activity, with use of a new substrate, Procion Brilliant Red M-2BS-Amylopectin. After 0.2 ml of serum or urine is incubated withExpand
Colorimetry of carbohydrates with m-aminophenol, and its proposed use in measurement of serum amylase activity.
  • J. Goodwin
  • Chemistry, Medicine
  • Clinical chemistry
  • 1976
TLDR
The relatively greater intensity of the m-aminophenol/maltose reaction product was used in a saccharogenic method for amylase, with low blank readings, and this method is compared with two dye/starch procedures. Expand
...
1
2
3
4
5
...