Modifications of laccase activities of copper efflux oxidase, CueO by synergistic mutations in the first and second coordination spheres of the type I copper center.

@article{Kataoka2013ModificationsOL,
  title={Modifications of laccase activities of copper efflux oxidase, CueO by synergistic mutations in the first and second coordination spheres of the type I copper center.},
  author={Kunishige Kataoka and Hiroki Kogi and Seiya Tsujimura and Takeshi Sakurai},
  journal={Biochemical and biophysical research communications},
  year={2013},
  volume={431 3},
  pages={
          393-7
        }
}
The redox potential of type I copper in the Escherichia coli multicopper oxidase CueO was shifted in the positive or negative direction as a result of the single, double, and triple mutations in the first and second coordination spheres: the formation of the NH···S(-)(Cys500 ligand) hydrogen bond, the breakdown of the NH(His443 ligand)···O(-)(Asp439) hydrogen bond, and the substitution of the Met510 ligand for the non-coordinating Leu or coordinating Gln. Laccase activities of CueO were… CONTINUE READING
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