Modifications of U2 snRNA are required for snRNP assembly and pre-mRNA splicing.

@article{Yu1998ModificationsOU,
  title={Modifications of U2 snRNA are required for snRNP assembly and pre-mRNA splicing.},
  author={Yi-Tao Yu and M. F. Shu and J. A. Steitz},
  journal={The EMBO journal},
  year={1998},
  volume={17 19},
  pages={5783-95}
}
Among the spliceosomal snRNAs, U2 has the most extensive modifications, including a 5' trimethyl guanosine (TMG) cap, ten 2'-O-methylated residues and 13 pseudouridines. At short times after injection, cellularly derived (modified) U2 but not synthetic (unmodified) U2 rescues splicing in Xenopus oocytes depleted of endogenous U2 by RNase H targeting. After prolonged reconstitution, synthetic U2 regenerates splicing activity; a correlation between the extent of U2 modification and U2 function in… CONTINUE READING

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