Modification of the triplet repeat primed polymerase chain reaction method for detection of the CTG repeat expansion in myotonic dystrophy type 1: application in preimplantation genetic diagnosis.

OBJECTIVE To overcome problems associated with the use of triplet repeat primed polymerase chain reaction (TP-PCR) in preimplantation genetic diagnosis (PGD) of myotonic dystrophy type 1 (DM1). DESIGN Clinical research study. SETTING UCL Centre for PGD and Centre for Reproductive and Genetic Health. PATIENT(S) Seven couples undergoing PGD for DM1… CONTINUE READING