Modification of human serum albumin with N-(2,5-dinitro-4-fluorophenyl)-4-amino-2,2,6,6-tetramethyl-piperidinooxy radical.

@article{Gerig1979ModificationOH,
  title={Modification of human serum albumin with N-(2,5-dinitro-4-fluorophenyl)-4-amino-2,2,6,6-tetramethyl-piperidinooxy radical.},
  author={John T Gerig and J D Reinheimer and Richard O Robinson},
  journal={Biochimica et biophysica acta},
  year={1979},
  volume={579 2},
  pages={409-20}
}
Human serum albumin has been treated with the spin-labeling reagent indicated in the title. Ultraviolet spectral studies of the protein so modified suggest that reaction takes place at lysine and tyrosine sidechains; kinetic experiments indicate that there are two especially reactive amino groups of the protein which are preferentially modified. Evidence is… CONTINUE READING