Modification of histidine biosynthesis pathway genes and the impact on production of l-histidine in Corynebacterium glutamicum

  title={Modification of histidine biosynthesis pathway genes and the impact on production of l-histidine in Corynebacterium glutamicum},
  author={Yongsong Cheng and Yunjiao Zhou and Lei Yang and Chenglin Zhang and Qing-yang Xu and Xixian Xie and Ning Chen},
  journal={Biotechnology Letters},
Histidine biosynthesis in Corynebacterium glutamicum is regulated not only by feedback inhibition by the first enzyme in the pathway, but also by repression control of the synthesis of the histidine enzymes. C. glutamicum histidine genes are located and transcribed in two unlinked loci, hisEG and hisDCB-orf1-orf2-hisHA-impA-hisFI. We constructed plasmid pK18hisDPtac to replace the native hisD promoter with the tac promoter, and overexpressed phosphoribosyl-ATP-pyrophosphohydrolase, encoded by… Expand
Corynebacterium glutamicum ATP-phosphoribosyl transferases suitable for L-histidine production--Strategies for the elimination of feedback inhibition.
L-Histidine biosynthesis in Corynebacterium glutamicum is mainly regulated by L-histidine feedback inhibition of the ATP-phosphoribosyltransferase HisG that catalyzes the first step of the pathway.Expand
Modular systems metabolic engineering enables balancing of relevant pathways for l-histidine production with Corynebacterium glutamicum
An intrinsically low ATP regeneration capacity is identified which prevents to maintain a balanced energy state of the cell in an l-histidine overproduction scenario and an insufficient supply of C1 units, which constitutes a general approach to improve the production of ATP and/or C1 intensive products. Expand
Transcriptional Regulation of the β-Type Carbonic Anhydrase Gene bca by RamA in Corynebacterium glutamicum
Evidence is presented that RamA negatively regulates expression of bca in C. glutamicum, and the transcriptional start site (TSS) of the bca gene was shown to be the first nucleotide “A” of its putative translational start codon (ATG) and thus, bca codes for a leaderless transcript. Expand
The RamA regulon: complex regulatory interactions in relation to central metabolism in Corynebacterium glutamicum
This review summarizes the current knowledge on RamA, its regulon, and its regulatory interplay with other transcriptional regulators coordinating the metabolism of C. glutamicum. Expand
Kinetics and Structure of a Cold-Adapted Hetero-Octameric ATP Phosphoribosyltransferase.
Rec recombinant HisGS and HisZ from the psychrophilic bacterium Psychrobacter arcticus were independently overexpressed and purified and Steady-state kinetics indicate that both the ATPPRT holoenzyme and HisGS are catalytically active. Expand
L-histidine production method and special recombinant bacteria
The invention discloses an L-histidine production method and special recombinant bacteria. The present invention provides a method to construct the recombinant bacteria, and the method includes theExpand
Identifying the Growth Modulon of Corynebacterium glutamicum
Central metabolism was observed to be regulated by a combination of metabolic and transcriptional activities orchestrating control over glycolysis, pentose phosphate pathway, and the tricarboxylic acid cycle. Expand
Modification of Corynebacterium glutamicum YILW for Isoleucine Production Improvement
To improve isoleucine production, ilvA was overexpressed in isoleucine producer Corynebacterium glutamicum YILW driven by promoter of tuf gene, which made no contribution to isoleucine improvementExpand
Allosteric Regulation of the First Enzyme in Histidine Biosynthesis
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Transcriptional regulation of histidine biosynthesis genes in Corynebacterium glutamicum.
Corynebacterium glutamicum, a gram-positive bacterium, has been widely used for industrial amino acid production. Corynebacterium glutamicum his genes are located and transcribed in two unlinkedExpand
Cloning of the ATP phosphoribosyl transferase gene of Corynebacterium glutamicum and application of the gene to L-histidine production.
Results indicate that the yield improvement results from amplification of the highly desensitized PRT provided by pCH99, which was derived from pCH13, had a larger production rate than F81 even in its production phase. Expand
Genetic and biochemical characterization of Corynebacterium glutamicum ATP phosphoribosyltransferase and its three mutants resistant to feedback inhibition by histidine.
The results demonstrated that the polarity change of electrostatic potential of mutant protein surface prevents histidine from binding to the C-terminal domain of HisG(Cg), resulting in the release of allosteric inhibition. Expand
Attenuation regulation of amino acid biosynthetic operons in proteobacteria: comparative genomics analysis.
Candidate attenuators were identified that regulate operons responsible for biosynthesis of branched amino acids, histidine, threonine, tryptophan, and phenylalanine in gamma- andExpand
Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon
The amounts of the ilvBNC and ilvNC transcripts increased in response to the addition of alpha-ketobutyrate to the growth medium, correlated to an increase in specific AHAS activity, whereas IR activity was not increased because of the relatively large amount of theIlvC transcript present under all conditions assayed. Expand
Feedback-resistant Phosphoribosyl-ATP Pyrophosphorylase in L-Histidine-producing Mutants of Corynebacterium glutarnicum
Phosphoribosyl-ATP pyrophosphorylase of KY-10522 is an improved strain in l-histidine-productivity through the additions of resistance markers including increased resistance to TRA, and is resistant to the feedback inhibition, like it’s parent strain. Expand
Pyruvate carboxylase as an anaplerotic enzyme in Corynebacterium glutamicum.
Results indicate that PCx is an essential anaplerotic enzyme for growth on glucose in the absence of PEPCx, PCx has no anaplerosis significance for grow on acetate as the carbon source, and PCX is anessential anaplerotics enzyme forgrowth on lactate even in the presence ofPEPCx. Expand
Histidine Production by Coiynebacterium glutamaicum Mutants, Multiresistant to Analogs of Histidine, Tryptophan, Purine and Pyrimidine
The histidine productivity of 1,2,4-triazole-3-alanine (TRA)-resistant histidine producer, Corynebacterium glutamicum KY–10260 was improved by successive additions of such markers as purine,Expand
β-Carotene production by Saccharomyces cerevisiae with regard to plasmid stability and culture media
Time course experiments demonstrated high plasmid stability even over extended cultivation periods, and growth experiments of a specific erg12 deletion mutant showed that the mevalonate kinase (MvaK1) was able to complement the function of the deleted native meValonate Kinase (Erg12) from S. cerevisiae in the MVA pathway under control of the constitutive adh1 promoter. Expand
Effect of feeding strategy on l-tryptophan production by recombinant Escherichia coli
It was found that the maximum specific growth rate and glucose concentration should be controlled at a low level in l-tryptophan production, which suggested a combined feeding strategy of pseudo-exponential feeding and glucose-stat feeding for optimal l-Tryptophans production. Expand