Modification of histidine biosynthesis pathway genes and the impact on production of l-histidine in Corynebacterium glutamicum

  title={Modification of histidine biosynthesis pathway genes and the impact on production of l-histidine in Corynebacterium glutamicum},
  author={Yongsong Cheng and Yunjiao Zhou and Lei Yang and Chenglin Zhang and Qing-yang Xu and Xixian Xie and Ning Chen},
  journal={Biotechnology Letters},
Histidine biosynthesis in Corynebacterium glutamicum is regulated not only by feedback inhibition by the first enzyme in the pathway, but also by repression control of the synthesis of the histidine enzymes. C. glutamicum histidine genes are located and transcribed in two unlinked loci, hisEG and hisDCB-orf1-orf2-hisHA-impA-hisFI. We constructed plasmid pK18hisDPtac to replace the native hisD promoter with the tac promoter, and overexpressed phosphoribosyl-ATP-pyrophosphohydrolase, encoded by… 

Highly efficient production of L-histidine from glucose by metabolically engineered Escherichia coli.

The engineering of wild-type Escherichia coli to overproduce histidine from glucose using metabolic strategies utilized here can be applied to engineering other microorganisms for the industrial production of histidine and related bioproducts.

Ecofriendly Synthesis of l-Carnosine in Metabolically Engineered Corynebacterium glutamicum by Reinforcing Precursor Accumulation.

A new biosynthetic process for l-carnosine is suggested and showed potential as a treatment for metabolic disorders through the assessment of its functions.

Modular systems metabolic engineering enables balancing of relevant pathways for l-histidine production with Corynebacterium glutamicum

An intrinsically low ATP regeneration capacity is identified which prevents to maintain a balanced energy state of the cell in an l-histidine overproduction scenario and an insufficient supply of C1 units, which constitutes a general approach to improve the production of ATP and/or C1 intensive products.

Transcriptional Regulation of the β-Type Carbonic Anhydrase Gene bca by RamA in Corynebacterium glutamicum

Evidence is presented that RamA negatively regulates expression of bca in C. glutamicum, and the transcriptional start site (TSS) of the bca gene was shown to be the first nucleotide “A” of its putative translational start codon (ATG) and thus, bca codes for a leaderless transcript.

The RamA regulon: complex regulatory interactions in relation to central metabolism in Corynebacterium glutamicum

This review summarizes the current knowledge on RamA, its regulon, and its regulatory interplay with other transcriptional regulators coordinating the metabolism of C. glutamicum.

Efficient Synthesis of Food-Derived Antioxidant l-Ergothioneine by Engineered Corynebacterium glutamicum.

This study presents the first strategy for EGT synthesis that does not require precursor addition in C. glutamicum, and the production time was shortened and the synthesized EGT showed high radical scavenging activity, thus confirming its antioxidant function.

L-histidine production method and special recombinant bacteria

The beneficial effects are that an new L-histidine fermentation yield improvement method is disclosed and proved, the effects of stack to increase yield is observed, and in practice, the L- historicalidine production method can be used for bacterial fermentation production of L- histidine.

Identifying the Growth Modulon of Corynebacterium glutamicum

Central metabolism was observed to be regulated by a combination of metabolic and transcriptional activities orchestrating control over glycolysis, pentose phosphate pathway, and the tricarboxylic acid cycle.

Modification of Corynebacterium glutamicum YILW for Isoleucine Production Improvement

To improve isoleucine production, ilvA was overexpressed in isoleucine producer Corynebacterium glutamicum YILW driven by promoter of tuf gene, which made no contribution to isoleucine improvement

Improving the Microbial Production of Amino Acids: From Conventional Approaches to Recent Trends

This proposed review aims to summarize traditional and recent strains in the microbial production of amino acids, characterize their metabolic pathways and present potential objectives for rational evolution.



Transcriptional regulation of histidine biosynthesis genes in Corynebacterium glutamicum.

Corynebacterium glutamicum, a gram-positive bacterium, has been widely used for industrial amino acid production. Corynebacterium glutamicum his genes are located and transcribed in two unlinked

Attenuation regulation of amino acid biosynthetic operons in proteobacteria: comparative genomics analysis.

Candidate attenuators were identified that regulate operons responsible for biosynthesis of branched amino acids, histidine, threonine, tryptophan, and phenylalanine in gamma- and

Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon

The amounts of the ilvBNC and ilvNC transcripts increased in response to the addition of alpha-ketobutyrate to the growth medium, correlated to an increase in specific AHAS activity, whereas IR activity was not increased because of the relatively large amount of theIlvC transcript present under all conditions assayed.

Feedback-resistant Phosphoribosyl-ATP Pyrophosphorylase in L-Histidine-producing Mutants of Corynebacterium glutarnicum

Phosphoribosyl-ATP pyrophosphorylase of KY-10522 is an improved strain in l-histidine-productivity through the additions of resistance markers including increased resistance to TRA, and is resistant to the feedback inhibition, like it’s parent strain.

Pyruvate carboxylase as an anaplerotic enzyme in Corynebacterium glutamicum.

Results indicate that PCx is an essential anaplerotic enzyme for growth on glucose in the absence of PEPCx, PCx has no anaplerosis significance for grow on acetate as the carbon source, and PCX is anessential anaplerotics enzyme forgrowth on lactate even in the presence ofPEPCx.

Histidine Production by Coiynebacterium glutamaicum Mutants, Multiresistant to Analogs of Histidine, Tryptophan, Purine and Pyrimidine

The histidine productivity of 1,2,4-triazole-3-alanine (TRA)-resistant histidine producer, Corynebacterium glutamicum KY–10260 was improved by successive additions of such markers as purine,

β-Carotene production by Saccharomyces cerevisiae with regard to plasmid stability and culture media

Time course experiments demonstrated high plasmid stability even over extended cultivation periods, and growth experiments of a specific erg12 deletion mutant showed that the mevalonate kinase (MvaK1) was able to complement the function of the deleted native meValonate Kinase (Erg12) from S. cerevisiae in the MVA pathway under control of the constitutive adh1 promoter.

Effect of feeding strategy on l-tryptophan production by recombinant Escherichia coli

It was found that the maximum specific growth rate and glucose concentration should be controlled at a low level in l-tryptophan production, which suggested a combined feeding strategy of pseudo-exponential feeding and glucose-stat feeding for optimal l-Tryptophans production.

Identification, nucleotide sequence and expression of the regulatory region of the histidine operon of Escherichia coli K-12.

Another promoter was identified on the same fragment of deoxyribonucleic acid by in vitro transcription, DNA sequencing and RNA polymerase binding and both promoters are very A-T rich and are separated by a G-C rich region containing a palyndromic structure.