Modification of aldose reductase by S-nitrosoglutathione.

@article{Chandra1997ModificationOA,
  title={Modification of aldose reductase by S-nitrosoglutathione.},
  author={Ak Chandra and Somya Srivastava and Jeffrey M. Petrash and Anshu Bhatnagar},
  journal={Biochemistry},
  year={1997},
  volume={36 50},
  pages={
          15801-9
        }
}
Kinetic and structural changes in recombinant human aldose reductase (AR) due to modification by S-nitrosoglutathione (GSNO) were investigated. Incubation of the enzyme with 10-50 microM GSNO led to a time- and concentration-dependent inactivation of the enzyme, with a second-order rate constant of 0.087 +/- 0.009 M-1 min-1. However, upon exhaustive modification, 30-40% of the enzyme activity was retained. The non-inactivated enzyme displayed a 2-3-fold change in Km for NADPH and Km fordl… CONTINUE READING
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