Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components

@article{Porebski1997ModificationOA,
  title={Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components},
  author={Sue Porebski and Lawrence G. Bailey and Bernard R. Baum},
  journal={Plant Molecular Biology Reporter},
  year={1997},
  volume={15},
  pages={8-15}
}
A relatively quick, inexpensive and consistent protocol for extraction of DNA from expanded leaf material containing large quantities of polyphenols, tannins and polysaccharides is described. Mature strawberry leaves, which contain high levels of these secondary components, were used as a study group. The method involves a modified CTAB extraction, employing high salt concentrations to remove polysaccharides, the use of polyvinyl pyrrolidone (PVP) to remove polyphenols, an extended RNase… CONTINUE READING

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