Modification of PCR conditions and design of exon-specific primers for the efficient molecular diagnosis of PKD1 mutations.

@article{Liu2014ModificationOP,
  title={Modification of PCR conditions and design of exon-specific primers for the efficient molecular diagnosis of PKD1 mutations.},
  author={Weiqiang Liu and Min Chen and Jia Hua Wei and Wenyin He and Zhihua Li and Xiaofang Sun and Yuling Shi},
  journal={Kidney & blood pressure research},
  year={2014},
  volume={39 6},
  pages={536-45}
}
BACKGROUND/AIMS Autosomal-dominant polycystic kidney disease (ADPKD) is a heterogeneous genetic disorder caused by mutations in the PKD1 and PKD2 genes. Currently, long-range PCR followed by nested PCR and sequencing (LRNS) is the gold standard approach for PKD1 testing. However, LRNS is complicated by the high structural and sequence complexity of PKD1… CONTINUE READING