Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene.

@article{Knutzon1992ModificationOB,
  title={Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene.},
  author={Deborah S. Knutzon and Gregory A. Thompson and Sharon E. Radke and Whitman B Johnson and Vic C. Knauf and Jean C. Kridl},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 7},
  pages={2624-8}
}
Molecular gene transfer techniques have been used to engineer the fatty acid composition of Brassica rapa and Brassica napus (canola) oil. Stearoyl-acyl carrier protein (stearoyl-ACP) desaturase (EC 1.14.99.6) catalyzes the first desaturation step in seed oil biosynthesis, converting stearoyl-ACP to oleoyl-ACP. Seed-specific antisense gene constructs of B. rapa stearoyl-ACP desaturase were used to reduce the protein concentration and enzyme activity of stearoyl-ACP desaturase in developing… CONTINUE READING

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