Modes of genotoxicity of a macromolecular antibiotic, SN-07, a novel type of interstrand DNA cross-linker.

  title={Modes of genotoxicity of a macromolecular antibiotic, SN-07, a novel type of interstrand DNA cross-linker.},
  author={N. Yajima and S. Ishida and N. Miyata and T. Kishi and G. Kawanishi},
  journal={Mutation research},
  volume={210 1},
The modes of genotoxicity of a novel macromolecular antitumor antibiotic (SN-07) were examined using both prokaryotic and eukaryotic cells in vitro. The antibiotic induced a frameshift-type reverse mutation in Ames Salmonella typhimurium TA98 at 1.6-400 ng/plate with and without S9 mix. SN-07 also induced chromosomal aberrations and a forward mutation (6-TGr) in Chinese hamster V79 cells after 1 h treatment at 12.5-100 ng/ml without metabolic activation. The alkaline elution technique revealed… Expand
DNA breaks and repair in the mouse leukemia L1210 cells exposed to three different types of interstrand DNA cross-linkers.
DNA breaks and repair in mouse leukemia L1210 cells treated with 3 different types of cross-linkers, mitomycin C (MMC), 1-(4-amino-2-methyl-5-pyrimidinyl)-methyl-3-(2-chloroethyl)-3-nitroso ure aExpand
Barminomycin, a model for the development of new anthracyclines.
Barminomycin is a member of the anthracycline class of anticancer agents and was originally discovered as a pink/red complex with DNA and RNA and named SN-07. The chromophore was subsequentlyExpand
Barminomycin forms GC-specific adducts and virtual interstrand crosslinks with DNA.
It was found that this compound formed blockages to transcription, and these blocks were highly selective for 5'-GC sequences, and barminomycin was also shown to form DNA virtual (i.e. functional) interstrand crosslinks. Expand


Mechanisms of action of a novel macromolecular antitumor antibiotic, SN-07, on mouse leukemia L1210 cells in culture.
The mechanisms of action of a novel macromolecular antitumor antibiotic (SN-07) were examined using cultured mouse lymphoid leukemia L1210 cells and typical inhibition of the cell cycle progression was observed at a cytocidal concentration of 25 ng/ml. Expand
Effect of DNA Repair systems on antibacterial and mutagenic activity of an antitumor protein, neocarzinostatin.
The results suggest that NCS produces hardly excisable DNA damage which is repaired by an error-prone recombination process. Expand
DNA strand scission by the antitumor protein neocarzinostatin.
Strand breaks are detected in both neutral and alkaline sucrose gradients, indicating that drug susceptibility is not due to alkali-labile bonds. Expand
Measurements of DNA damage in Chinese hamster cells treated with equitoxic and equimutagenic doses of nitrosoureas.
Data suggest that the ability to cross-link DNA confers increased cytotoxicity to the haloethylnitrosoureas. Expand
Auromomycin-induced DNA damage and repair in human leukemic lymphoblasts (CCRF-CEM cells).
The auromomycin-induced single strand breaks were repaired to almost completion within 8 h of postincubation of DNA-damaged cells whereas the repair of double strand breaks was not detected. Expand
DNA crosslinking induced by x-rays and chemical agents.
Evidence is presented that crossl linking occurs immediately after X-irradiation and is repaired with incubation at 37°C, and X-ray crosslinking was attributed to DNA-protein crosslinks since treatment of the cell lysates with proteinase removed the effect. Expand
Genetics of Human Cell Lines I. 8-Azaguanine Resistance, a Selective “Single-Step” Marker.∗
  • W. Szybalski, M. Smith
  • Biology, Medicine
  • Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1959
Summary A cloned wild-type population (strain D98S) derived from human bone marrow cells, which is inhibited by 8-azaguanine (AG) in concentrations in excess of 0.1 μg/ml, contains approximately 1 toExpand
Mutagenesis by chemical agents in V79 chinese hamster cells: a review and analysis of the literature. A report of the Gene-Tox Program.
The report reviews and evaluates the current literature on chemically induced specific locus mutations in the V79 Chinese hamster lung cell line, and discusses the protocols for quantitative mutation studies including measurements of cytotoxicity, mutant expression times, mutant selection agents, cell densities during selection, and the stability and verification of mutant phenotypes. Expand
Mutagenicity of several classes of antitumor agents to Salmonella typhimurium TA98, TA100, and TA92.
Four antitumor agents, including busulfan, carbazilquinone, 1-(4-amino-2-methylpyrimidine-5-yl)methyl-3-(2-chloroethyl)-3-nitrosourea hydrochloride, and pipobroman were shown for the first time to be mutagenic. Expand
Reverse mutation tests in Salmonella typhimurium and chromosomal aberration tests in mammalian cells in culture on fluorinated pyrimidine derivatives.
All compounds tested, except uracil, induced chromosomal aberrations on CHL cells in the system without metabolic activation, and FD-1 showed the strongest anti-bacterial action when S9 mix was present but it was degraded by S9, while UFT showed no anti-Bacterial action in any conditions. Expand