Modern tools for identification of nucleic acid-binding proteins.

  title={Modern tools for identification of nucleic acid-binding proteins.},
  author={Nadia H{\'e}garat and Jean-Christophe François and Dani{\`e}le Praseuth},
  volume={90 9},
Numerous biological mechanisms depend on nucleic acid--protein interactions. The first step to the understanding of these mechanisms is to identify interacting molecules. Knowing one partner, the identification of other associated molecular species can be carried out using affinity-based purification procedures. When the nucleic acid-binding protein is known, the nucleic acid can be isolated and identified by sensitive techniques such as polymerase chain reaction followed by DNA sequencing or… CONTINUE READING

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