Modelling the limit of detection in real-time quantitative PCR

@article{Burns2008ModellingTL,
  title={Modelling the limit of detection in real-time quantitative PCR},
  author={M. Burns and Harold Valdivia},
  journal={European Food Research and Technology},
  year={2008},
  volume={226},
  pages={1513-1524}
}
The limit of detection (LOD) is a critical performance characteristic of an assay that requires careful evaluation during method validation. However, formal calculations for the LOD do not take into account atypical data sets that are generated from real-time PCR techniques, which can be non-normally distributed, truncated, and heteroscedastic. Experimental data sets for the quantification of genetically modified (GM) material were produced using real-time PCR, in order to model the LOD. A… CONTINUE READING

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