Modelling the hair follicle dermal papilla using spheroid cell cultures

@article{Higgins2010ModellingTH,
  title={Modelling the hair follicle dermal papilla using spheroid cell cultures},
  author={Claire A. Higgins and Gavin D. Richardson and D Ferdinando and Gillian E. Westgate and Colin A B Jahoda},
  journal={Experimental Dermatology},
  year={2010},
  volume={19}
}
Please cite this paper as: Modelling the hair follicle dermal papilla using spheroid cell cultures. Experimental Dermatology 2010; 19: 546–548. 
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The isolation of four dermal fibroblast populations from human haired skin and their maintenance in culture is described.
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The micro‐dissection and culture methods, which are used within the laboratory, for the study of human dermal papilla cells are described.
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Characteristics such as formation of hair shafts and sebaceous glands, presence of hair follicles with features of anagen or telogen follicles, and reproducible hair and skin function parameters make this model a tool to study human hair follicle neogenesis and development.
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The authors' 3D skin equivalent culture assay reproducibly regenerated hair follicles, while MDCs precultured in the 2D model with MECs did not, and several representative genes associated with hair induction showed higher expression in their assay than in the chamber assay.
Human hair follicle dermal sheath and papilla cells support keratinocyte growth in monolayer coculture
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The capacity of human hair follicle dermal cells to act as alternative supports for keratinocyte growth is investigated and secreted protein acidic and rich in cysteine (SPARC) expression increased DS and DP cells relative to DFs; however, further experiments did not demonstrate a role in keratinocytes support.
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References

SHOWING 1-10 OF 19 REFERENCES
The growth of vibrissa dermal papilla cells in vitro
TLDR
Initially the vibrissa cells demonstrated singular characteristics, including poor spreading ability, before eventually forming a fibroblast‐like population of cells, which is compared with cultured feather dermal papillae.
The in vitro properties of dermal papilla cell lines established from human hair follicles
TLDR
In serial subcultures, the dermal papilla cells displayed a spread out, polygonal cellular morphology at stationary growth phases and a tendency to form multi‐layered aggregates before reaching confluence, compared with dermal fibroblasts which exhibited a shorter in vitro survival time.
Induction of hair growth by implantation of cultured dermal papilla cells
TLDR
It is reported here that serially cultured adult papilla cells can induce the growth of hair when implanted into follicles which otherwise would not grow hairs.
Plasticity and cytokinetic dynamics of the hair follicle mesenchyme: implications for hair growth control.
TLDR
The hair follicle mesenchyme exhibits significant hair cycle-associated plasticity, and modulation of these cell interchanges is likely to be important during clinically importanthair follicle transformations, e.g. vellus-to-terminal and terminal- to-vellus during androgenetic alopecia.
Wnt signaling maintains the hair-inducing activity of the dermal papilla.
TLDR
It is demonstrated that specific Wnts, but not Sonic hedgehog (Shh), maintain anagen-phase gene expression in vitro and hair inductive activity in a skin reconstitution assay.
Vibrissa dermal papilla cell aggregative behaviour in vivo and in vitro.
TLDR
The collective behaviour of the dermal papilla cells revealed in this study may be significant for the morphogenetic activity of the papilla, and for papilla size during the hair cycle, and may also reflect the retention of embryonic-like properties by the Dermal component of adult hair follicles.
Cultured peribulbar dermal sheath cells can induce hair follicle development and contribute to the dermal sheath and dermal papilla.
TLDR
The results indicate transplanted DP and DSC cells were equally capable of DP formation and hair follicle induction, and suggests the DP and peribulbar DSC may be functionally similar.
Long-term culture of mouse vibrissal dermal papilla cells and de novo hair follicle induction.
TLDR
It is suggested that FGF-2 is essential for dermal papilla cell culture and that sphere formation partially models the intact DP, resulting in hair follicle induction, even by later passaged cells.
Whisker growth induced by implantation of cultured vibrissa dermal papilla cells in the adult rat.
TLDR
It is demonstrated that cultured papilla cells of early passage numbers retain their ability to induce hair growth, which appears to be an essential functional component of papilla cell activity which operates to regulate the profound morphogenetic changes that occur during the hair growth cycle.
Selective activation of the versican promoter by epithelial- mesenchymal interactions during hair follicle development.
TLDR
It is demonstrated that a short fragment of the human versican promoter is sufficient to activate lacZ reporter gene expression in the DP of postnatal transgenic mice and also in the condensed mesenchyme beneath the hair placode during hair follicle embryogenesis, suggesting that versican may play an essential role both in mesenchymal condensation and in hair induction.
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