Modeling with in vitro kinetic parameters for the elaboration of transfer RNA identity in vivo.

Abstract

A tRNA with "double identity" was created, and this tRNA was demonstrated in vitro to aminoacylate quantitatively with either of two amino acids. In contrast, acceptance of only one of these amino acids was observed in vivo, and a simple manipulation determined which one was accepted. Kinetic parameters were obtained for aminoacylation with each amino acid… (More)

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@article{Hou1989ModelingWI, title={Modeling with in vitro kinetic parameters for the elaboration of transfer RNA identity in vivo.}, author={Y. M. Hou and Priscilla Schimmel}, journal={Biochemistry}, year={1989}, volume={28 12}, pages={4942-7} }