Modeling the structure of the type I peritrophic matrix: characterization of a Mamestra configurata intestinal mucin and a novel peritrophin containing 19 chitin binding domains.

@article{Shi2004ModelingTS,
  title={Modeling the structure of the type I peritrophic matrix: characterization of a Mamestra configurata intestinal mucin and a novel peritrophin containing 19 chitin binding domains.},
  author={Xianzong Shi and Mahmood Chamankhah and Savita Visal-Shah and Sean M. Hemmingsen and Martin Erlandson and Lorraine Braun and Michelle A Alting-Mees and George G. Khachatourians and Michael O'grady and Dwayne D Hegedus},
  journal={Insect biochemistry and molecular biology},
  year={2004},
  volume={34 10},
  pages={1101-15}
}
Twelve to fourteen integral proteins were found to reside in the Type I peritrophic matrix (PM) of Mamestra configurata (bertha armyworm) larvae. Several methods were employed, including de novo peptide sequencing, the generation of a midgut-specific EST database and immunological screening, which led to the isolation of cDNAs encoding two integral PM proteins. McPM1, the largest PM protein described to date at 202 kDa, was comprised of a concatamer of 19 chitin binding domains (CBD), 12 of… CONTINUE READING