• Corpus ID: 211011152

Modeling supercoiled DNA interacting with an anchored cluster of proteins: towards a quantitative estimation of chromosomal DNA supercoiling

@article{Walter2020ModelingSD,
  title={Modeling supercoiled DNA interacting with an anchored cluster of proteins: towards a quantitative estimation of chromosomal DNA supercoiling},
  author={Jean-Charles Walter and Thibaut Lepage and J{\'e}r{\^o}me Dorignac and Fr{\'e}d{\'e}ric Geniet and Andrea Parmeggiani and John Palmeri and Jean-Yves Bouet and Ivan Junier},
  journal={arXiv: Biological Physics},
  year={2020}
}
We investigate the measurement of DNA supercoiling density ($\sigma$) along chromosomes using interaction frequencies between DNA and DNA-anchored clusters of proteins. Specifically, we show how the physics of DNA supercoiling leads, in bacteria, to the quantitative modeling of binding properties of ParB proteins around their centromere-like site, {\it parS}. Using this framework, we provide an upper bound for $\sigma$ in the {\it Escherichia coli} chromosome, consistent with plasmid values… 

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Modeling the formation and positioning of intracellular macromolecular assemblies: Application to bacterial DNA segregation

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