Modeling and Robustness Analysis of Biochemical Networks of Glycerol Metabolism by Klebsiella Pneumoniae

  title={Modeling and Robustness Analysis of Biochemical Networks of Glycerol Metabolism by Klebsiella Pneumoniae},
  author={Jianxiong Ye and Enmin Feng and Lei Wang and Zhilong Xiu and Yaqin Sun},
Glycerol bioconversion to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae (K. pneumoniae) can be characterized by an intricate network of interactions among biochemical fluxes, metabolic compounds, key enzymes and genetic regulatory. To date, there still exist some uncertain factors in this complex network because of the limitation in bio-techniques, especially in measuring techniques for intracellular substances. In this paper, among these uncertain factors, we aim to infer the transport… 
Complex metabolic network of 1,3-propanediol transport mechanisms and its system identification via biological robustness
A 14-dimensional nonlinear hybrid dynamical system aiming to determine the most possible metabolic system and the corresponding optimal parameter is established and numerical results show that it is most possible that 1,3-PD passes the cell membrane by active transport coupled with passive diffusion.
Determining the transport mechanism of an enzyme-catalytic complex metabolic network based on biological robustness
  • Lei Wang
  • Computer Science
    Bioprocess and Biosystems Engineering
  • 2012
A system identification model is presented to infer the most possible transport mechanism of 1,3-PD, in which the performance index consists of the relative error of the extracellular substance concentrations and biological robustness of the intracellular substances concentrations.
Parameter identification of nonlinear delayed dynamical system in microbial fermentation based on biological robustness
The nonlinear enzyme-catalytic kinetic system of batch and continuous fermentation in the process of glycerol bio-dissimilation is investigated and the delay of concentration changes on both extracellular substances and intracellular substances is considered.
Modeling and identification of hybrid dynamic system in microbial continuous fermentation
A hybrid dynamic model using fuzzy expert system is investigated in the process of glycerol bioconversion to 1,3-PD by Klebsiella pneumoniae and indicates that proposed hybrid model adding fuzzy system is more appropriate and the optimization algorithm is effective.
In this paper, a hybrid dynamic model using fuzzy expert system is investigated in the process of glycerol bioconversion to 1,3-PD by Klebsiella pneumoniae(K.pneumoniae). In continuous culture, we


Comparative Genomic Analysis of dha Regulon and Related Genes for Anaerobic Glycerol Metabolism in Bacteria
In silico reconstruction and comparison of dha regulons revealed several new genes in the microorganisms studied, including a novel dha kinase that is phosphoenolpyruvate‐dependent is identified and experimentally confirmed for K. pneumoniae and explains some hitherto not well understood experimental observations.
Microbial production of 1,3-propanediol
Great efforts are now being made to combine the pathway from glucose to glycerol successfully with the bacterial route from Glycerol to 1,3-PD, which may become the first bulk chemical produced by a genetically engineered microorganism.
Robustness in simple biochemical networks
It is argued that the key properties of biochemical networks should be robust in order to ensure their proper functioning, and it is shown that this applies in particular to bacterial chemotaxis.
Cloning of 1,3-propanediol oxidoreductase gene from Klebsiella pneumoniae and preliminary study on its expression conditions
The results of SDS-Page analysis and protein localization assay reflected the high expression of PDOR in E.coli BL21(DE3) and the effect of induction temperature on enzyme activity was investigated, suggesting the distinct effect of temperature on protein expression.
Multiple product inhibition and growth modeling of clostridium butyricum and klebsiella pneumoniae in glycerol fermentation
The inhibition potentials of products and substrate on the growth ofClostridium butyricum and Klebsiella pneumoniae in the glycerol fermentation are examined from experimental data and with a
A kinetic model for product formation of microbial and mammalian cells
  • A. Zeng
  • Biology, Engineering
    Biotechnology and bioengineering
  • 1995
The Luedeking–Piret kinetic is extended to include a term describing the effect of residual substrate concentration, in which the product formation can be quantitatively assessed, and is demonstrated with three microbial cultures for the production of primary metabolites and three hybridoma cell cultures over a wide range of substrate concentration.
Robustness of Cellular Functions
Towards a theory of biological robustness
  • H. Kitano
  • Biology
    Molecular systems biology
  • 2007
The value of understanding fundamental and structural theories is that they provide deeper insights into the governing principles that complex evolvable systems including biological systems that define the possible design space of life.