A model for the chemomechanical coupling of the mammalian cytoplasmic dynein molecular motor
- BiologyEuropean Biophysics Journal
Based on this model, analytical studies of the motor dynamics are presented, indicating that under no or low force the motors exhibit a tight chemomechanical coupling, and as the force increases the number of ATPs consumed per step increases greatly.
Expression of dynein, cytoplasmic 2, heavy chain 1 (DHC2) associated with glioblastoma cell resistance to temozolomide
- BiologyScientific reports
Ex vivo data showed that DHC2 expression in GBM tissue samples was associated with tumor recurrence after TMZ chemotherapy, and results indicated cytoskeleton related protein D HC2 reduced sensitivity of GBM cells to TMZ treatment.
SHOWING 1-10 OF 28 REFERENCES
Multiscale approaches for studying energy transduction in dynein.
- BiologyPhysical chemistry chemical physics : PCCP
A structural model of dynein's motor unit showing a heptameric organization of the motor subunits is described and a kinetic model of the coordination between the two Dynein heads is presented.
Cytoplasmic dynein functions as a gear in response to load
This work uses an optical trap to quantify motion of polystyrene beads driven along microtubules by single cytoplasmic dynein motors and proposes a model whereby the gear is downshifted through load-induced binding of ATP at secondary sites in the Dynein head.
Cytoplasmic Dynein Moves Through Uncoordinated Stepping of the AAA+ Ring Domains
The data suggest that the two heads do not cooperate during movement, which suggests a fundamentally different mechanism of motility from that observed for other microtubule-based motors.
A simple theoretical model explains dynein's response to load.
- BiologyBiophysical journal
A simple model is proposed that provides a simple understanding on how a variable chemomechanical coupling ratio can be achieved and used to optimize the biological function of dynein.
A physical model reveals the mechanochemistry responsible for dynein's processive motion.
- BiologyBiophysical journal
Processive bidirectional motion of dynein–dynactin complexes in vitro
- Biology, ChemistryNature Cell Biology
It is shown that individual fluorescently labelled dynein–dynactin complexes exhibit bidirectional and processive motility towards both the plus and minus ends of microtubules, suggesting that bid Directional motility is an active energy-transduction property of dyneIn–dwnactin motor mechano-chemistry.
Kinetic models for the coordinated stepping of cytoplasmic dynein.
- BiologyThe Journal of chemical physics
This work develops and analyzes a simple mechanical model for the force-induced stepping in the absence of ATP and uses the results to develop a full model for dimeric dynein's chemomechanical cycle and analyze this model to make experimentally testable predictions.
Monte Carlo modeling of single-molecule cytoplasmic dynein.
- BiologyProceedings of the National Academy of Sciences of the United States of America
This model reproduces the large steps found experimentally under high ATP and no load by assuming that the ATP binding affinities at the secondary sites decrease as the number of ATP bound to these sites increases.