Model-driven engineering of RNA devices to quantitatively program gene expression.

@article{Carothers2011ModeldrivenEO,
  title={Model-driven engineering of RNA devices to quantitatively program gene expression.},
  author={James M Carothers and Jonathan A. Goler and Darmawi Juminaga and Jay D Keasling},
  journal={Science},
  year={2011},
  volume={334 6063},
  pages={1716-9}
}
The models and simulation tools available to design functionally complex synthetic biological devices are very limited. We formulated a design-driven approach that used mechanistic modeling and kinetic RNA folding simulations to engineer RNA-regulated genetic devices that control gene expression. Ribozyme and metabolite-controlled, aptazyme-regulated expression devices with quantitatively predictable functions were assembled from components characterized in vitro, in vivo, and in silico. The… CONTINUE READING
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