Model-based local density sharpening of cryo-EM maps

  title={Model-based local density sharpening of cryo-EM maps},
  author={Arjen J Jakobi and Matthias Wilmanns and Carsten Sachse},
Atomic models based on high-resolution density maps are the ultimate result of the cryo-EM structure determination process. Here, we introduce a general procedure for local sharpening of cryo-EM density maps based on prior knowledge of an atomic reference structure. The procedure optimizes contrast of cryo-EM densities by amplitude scaling against the radially averaged local falloff estimated from a windowed reference model. By testing the procedure using six cryo-EM structures of TRPV1… CONTINUE READING
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