Model-assisted fine-tuning of central carbon metabolism in yeast through dCas9-based regulation.

@article{Ferreira2019ModelassistedFO,
  title={Model-assisted fine-tuning of central carbon metabolism in yeast through dCas9-based regulation.},
  author={Raphael Ferreira and Christos Skrekas and Alex Hedin and Benjam{\'i}n J. S{\'a}nchez and Verena Siewers and Jens Nielsen and Florian David},
  journal={ACS synthetic biology},
  year={2019}
}
Engineering Saccharomyces cerevisiae for industrial-scale production of valuable chemicals involves extensive modulation of its metabolism. Here, we identified novel gene expression fine-tuning set-ups to enhance endogenous metabolic fluxes towards increasing levels of acetyl-CoA and malonyl-CoA. dCas9-based transcriptional regulation was combined together with a malonyl-CoA responsive intracellular biosensor to select for beneficial set-ups. The candidate genes for screening were predicted… CONTINUE READING