Mode of pectin deesterification byTrichoderma reesei pectinesterase

Trichoderma reesei pectinesterase (isoelectric point 8.3–9.5; pH-optimum 7.6) catalyzes deesterification of pectin by giving rise to blocks of free carboxyl groups in the macromolecule; a mode similar to that observed with higher plant pectinesterases which also have Ip and pH-optima in the alkaline region.