Mode of action of membrane-disruptive lytic compounds from the marine dinoflagellate Alexandrium tamarense.

@article{Ma2011ModeOA,
  title={Mode of action of membrane-disruptive lytic compounds from the marine dinoflagellate Alexandrium tamarense.},
  author={Haiyan Ma and Bernd Krock and Urban Tillmann and Ulf Bickmeyer and Martin Graeve and Allan D Cembella},
  journal={Toxicon : official journal of the International Society on Toxinology},
  year={2011},
  volume={58 3},
  pages={247-58}
}
Certain allelochemicals of the marine dinoflagellate Alexandrium tamarense cause lysis of a broad spectrum of target protist cells but the lytic mechanism is poorly defined. We first hypothesized that membrane sterols serve as molecular targets of these lytic compounds, and that differences in sterol composition among donor and target cells may cause insensitivity of Alexandrium and sensitivity of targets to lytic compounds. We investigated Ca(2+) influx after application of lytic fractions to… CONTINUE READING

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We investigated Ca(2 + ) influx after application of lytic fractions to a model cell line PC12 derived from a pheochromocytoma of the rat adrenal medulla to establish how the lytic compounds affect ion flux associated with lysis of target membranes .
We investigated Ca(2 + ) influx after application of lytic fractions to a model cell line PC12 derived from a pheochromocytoma of the rat adrenal medulla to establish how the lytic compounds affect ion flux associated with lysis of target membranes .
Adrenal MedullaIs normal tissue origin of diseasePheochromocytoma
We investigated Ca(2 + ) influx after application of lytic fractions to a model cell line PC12 derived from a pheochromocytoma of the rat adrenal medulla to establish how the lytic compounds affect ion flux associated with lysis of target membranes .
For three tested sterols , the lytic compounds showed highest affinity towards cholesterol followed by ergosterol and brassicasterol .
For three tested sterols , the lytic compounds showed highest affinity towards cholesterol followed by ergosterol and brassicasterol .
Cholesterol comprised a higher percentage of total sterols in plasma membrane fractions of A. tamarense than in corresponding whole cell fractions .
For three tested sterols , the lytic compounds showed highest affinity towards cholesterol followed by ergosterol and brassicasterol .
Analysis of sterol composition of isolates of A. tamarense and of five target protistan species showed that both lytic and non - lytic A. tamarense strains contain cholesterol and dinosterol as major sterols , whereas none of the other tested species contain dinosterol .
Cholesterol comprised a higher percentage of total sterols in plasma membrane fractions of A. tamarense than in corresponding whole cell fractions .
For three tested sterols , the lytic compounds showed highest affinity towards cholesterol followed by ergosterol and brassicasterol .
Analysis of sterol composition of isolates of A. tamarense and of five target protistan species showed that both lytic and non - lytic A. tamarense strains contain cholesterol and dinosterol as major sterols , whereas none of the other tested species contain dinosterol .
Analysis of sterol composition of isolates of A. tamarense and of five target protistan species showed that both lytic and non - lytic A. tamarense strains contain cholesterol and dinosterol as major sterols , whereas none of the other tested species contain dinosterol .
For three tested sterols , the lytic compounds showed highest affinity towards cholesterol followed by ergosterol and brassicasterol .
Cholesterol comprised a higher percentage of total sterols in plasma membrane fractions of A. tamarense than in corresponding whole cell fractions .
Certain allelochemicals of the marine dinoflagellate Alexandrium tamarense cause lysis of a broad spectrum of target protist cells but the lytic mechanism is poorly defined .
Adding sterols and phosphatidylcholine to a lysis bioassay with the cryptophyte Rhodomonas salina for evaluation of competitive binding indicated that the lytic compounds possessed apparent high affinity for free sterols and phosphatidylcholine .
We investigated Ca(2 + ) influx after application of lytic fractions to a model cell line PC12 derived from a pheochromocytoma of the rat adrenal medulla to establish how the lytic compounds affect ion flux associated with lysis of target membranes .
Certain allelochemicals of the marine dinoflagellate Alexandrium tamarense cause lysis of a broad spectrum of target protist cells but the lytic mechanism is poorly defined .
We investigated Ca(2 + ) influx after application of lytic fractions to a model cell line PC12 derived from a pheochromocytoma of the rat adrenal medulla to establish how the lytic compounds affect ion flux associated with lysis of target membranes .
We investigated Ca(2 + ) influx after application of lytic fractions to a model cell line PC12 derived from a pheochromocytoma of the rat adrenal medulla to establish how the lytic compounds affect ion flux associated with lysis of target membranes .
For three tested sterols , the lytic compounds showed highest affinity towards cholesterol followed by ergosterol and brassicasterol .
For three tested sterols , the lytic compounds showed highest affinity towards cholesterol followed by ergosterol and brassicasterol .
For three tested sterols , the lytic compounds showed highest affinity towards cholesterol followed by ergosterol and brassicasterol .
Certain allelochemicals of the marine dinoflagellate Alexandrium tamarense cause lysis of a broad spectrum of target protist cells but the lytic mechanism is poorly defined .
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