Mode of action of endoglucanase III from Trichoderma reesei.

  title={Mode of action of endoglucanase III from Trichoderma reesei.},
  author={Ricardo Macarron and Carmen Acebal and Mar{\'i}a Pilar Castill{\'o}n and Juan Manuel Dom{\'i}nguez and Isabel de la Mata and Goran Pettersson and Peter Tomme and Marc Claeyssens},
  journal={The Biochemical journal},
  volume={289 ( Pt 3)},
Endoglucanase III (EG III) was purified to homogeneity from the culture medium of Trichoderma reesei QM 9414. It has a molecular mass of 48 kDa, and an isoelectric point of 5.1. Maximal activity was observed between pH4 and 5. Celloligosaccharides and their chromophoric derivatives were used as substrates, and the reaction products were analysed by quantitative h.p.l.c. Nucleophilic competition experiments (between methanol and water) allowed unequivocal assessment of cleavage sites. EG III… CONTINUE READING