Mode of action of alanosine.

  title={Mode of action of alanosine.},
  author={Glen R. Gale and G. B. Schmidt},
  journal={Biochemical pharmacology},
  volume={17 3},
Abstract Alanosine inhibits the synthesis of RNA adenine from formate- 14 C and from glycine- 14 C in Ehrlich ascites tumor cells in vivo . There is no appreciable depression of the rate of RNA guanine synthesis. The incorporation of preformed adenine- 14 C into RNA purines is unaffected. Inhibition of RNA adenine synthesis is not antagonized by glutamine or aspartate. The incorporation of thymidine- 3 H, uridine- 3 H, and l -leucine- 14 C into the acid-insoluble fraction of the cells in vitro… Expand
Effects of alanosine on purine and pyrimidine synthesis.
It was tentatively concluded that the drug depresses the activity of aspartate transcarbamylase and adenylosuccinate synthetase and that the inhibition by the drug of the growth of the organism was antagonized by aspartates. Expand
Pharmacology of captan: biochemical effects with special reference to macromolecular synthesis.
Captan inhibits the synthesis of RNA purines-14 C from formate- 14 C in ascites tumor cells in vivo as well as in cultures of Candida albicans ; this action on tumor cells was demonstrated after intraperitoneal or oral administration of the inhibitor. Expand
A novel synergistic effect of alanosine and guanine on adenine nucleotide synthesis in mammalian cells. Alanosine as a useful probe for investigating purine nucleotide metabolism
  • R. Gupta
  • Biology, Medicine
  • Journal of cellular physiology
  • 1980
Results suggest that guanine nucleotides exert a regulatory effect on the activity of the enzyme adenylosuccinate synthetase, and indicates that alanosine provides a useful probe for investigating the regulation of purine nucleotide metabolism in mammalian cells. Expand
Alanosine toxicity in Novikoff rat hepatoma cells due to inhibition of the conversion of inosine monophosphate to adenosine monophosphate.
2-Amino-3-(hydroxynitrosoamino)propionic acid (alanosine), at a concentration as low as 2.7 muM, completely inhibits the incorporation of hypoxanthine into adenosine triphosphate by cultured NovikoffExpand
Adenine purine nucleotide cycle enzymes and 3' -methyl-4-dimethylaminoazobenzene injections.
Rat liver enzymes involved in the adenine purine nucleotide cycle were assayed for activity after intra-abdominal injections of the carcinogen 3′-Me-DAB and showed significant increases in activity within 2–4 days post injection, which may have significance in the ability to convert the normal liver into a state of malignancy. Expand
Modulation of fluoropyrimidine metabolism in L1210 cells by L-alanosine.
L-alanosine appeared to augment the RNA-directed activity of FUra and FUrd in that there was no correlation between the enhanced metabolism and cytotoxicity of these two fluoropyrimidines and either levels of fluorodeoxyuridylate (FdUMP) which inhibits thymidylATE (TMP) synthetase or inhibition of the ability of cells to incorporate deoxyurodylate into acid-precipitable material. Expand
Effect of L-alanosine on immune response.
In vivo treatment with L-alanosine does not impair spleen natural killer activity and the in vitro lymphoproliferative responses of splenocytes to Concanavalin A and bacterial Lipopolysaccharides are affected when the drug is added upon setting up the cultures. Expand
Contragestational profile of the tumor-inhibiting agent,L-alanosine, in the rat and the hamster
The ‘contragestational test’, i.e., the effect on conceptus growth, appears to be an interesting approach for learning more about the antiproliferative activity of an antineoplastic agent. Expand
Identification of the antimetabolite of L-alanosine, L-alanosyl-5-amino-4-imidazolecarboxylic acid ribonucleotide, in tumors and assessment of its inhibition of adenylosuccinate synthetase.
It was shown for the first time in vivo that the parenteral administration of L-alanosine reduces the specific activity of intratumoral adenylosuccinate synthetase by 70% and depresses the synthesis of DNA to an equivalent or greater extent; adenine but not hypoxanthine was able to reverse the latter inhibition. Expand
Use of alanosine as a methylthioadenosine phosphorylase-selective therapy for T-cell acute lymphoblastic leukemia in vitro.
The results support the use of L-alanosine alone or in combination with a salvage agent as aMTAP-selective therapy and lay the foundation for the initiation of clinical trials for the treatment of T-ALL and other MTAP-deficient malignancies with L-Alanosine. Expand


Studies on the mode of action of azaserine.
The results indicate that azaserine inhibits purine synthesis in the intact animal at a stage prior to the formation of 5-amino-4-imidazole-carboxamide ribotide. Expand
Biosynthesis of the purines. XV. The effect of aza-L-serine and 6-diazo-5-oxo-L-norleucine on inosinic acid biosynthesis de novo.
Detailed information is provided on the site and mechanism of the inhibitory act,ion by this compound, together with more recent data on the action of 6-diazo-5-oxo-n-norleucine on inosinic acid biosynthesis. Expand
Alanosine, a New Antiviral and Antitumour Agent isolated from a Streptomyces
A new antibiotic with antiviral and antitumour activity to which the name “alanosine” was given was given after it was isolated from a Brazilian soil sample. Expand
Enzymatic synthesis of adenosine-5'-phosphate from inosine-5'-phosphate.
  • I. Lieberman
  • Medicine, Biology
  • The Journal of biological chemistry
  • 1956