Mode of Expression and Functional Characterization of FCT-3 Pilus Region-Encoded Proteins in Streptococcus pyogenes Serotype M49

@article{Nakata2008ModeOE,
  title={Mode of Expression and Functional Characterization of FCT-3 Pilus Region-Encoded Proteins in Streptococcus pyogenes Serotype M49},
  author={Masanobu Nakata and Thomas Köller and Karin Moritz and Deborah A. Ribardo and Ludwig Jonas and Kevin S. McIver and Tomoko Sumitomo and Yutaka Terao and Shigetada Kawabata and Andreas Podbielski and Bernd Kreikemeyer},
  journal={Infection and Immunity},
  year={2008},
  volume={77},
  pages={32 - 44}
}
ABSTRACT The human pathogen Streptococcus pyogenes (group A streptococcus [GAS]) pilus components, suggested to play a role in pathogenesis, are encoded in the variable FCT (fibronectin- and collagen-binding T-antigen) region. We investigated the functions of sortase A (SrtA), sortase C2 (SrtC2), and the FctA protein of the most prevalent type 3 FCT region from a serotype M49 strain. Although it is considered a housekeeping sortase, SrtA's activity is involved in pilus formation in addition to… 
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
The current state of the art regarding GAS pili is summarized, including a new mode of host-GAS interaction mediated by pili, along with insights into pilus expression in terms of tissue tropism.
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TLDR
Since the highly virulent strains under investigation are unlikely to produce other pili, the results suggest that pili might be dispensable for critical steps of the S. suis pathogenesis of infection.
Structural Conservation, Variability, and Immunogenicity of the T6 Backbone Pilin of Serotype M6 Streptococcus pyogenes
TLDR
Although the GAS BP show large variations in size and sequence, the modular nature of the pilus proteins revealed by the T6 structure may aid the future design of a pilus-based vaccine.
Linkage of T3 and Cpa pilins in the Streptococcus pyogenes M3 pilus
TLDR
The residues involved in their linkage are investigated and it is shown that linkage of Cpa to T3 by the sortase family transpeptidase SrtC2 requires the VPPTG motif in the cell wall‐sorting signal of CPA, suggesting that Cpa is located exclusively at the pilus tip, a location supported by immunogold electron microscopy.
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TLDR
It is reported that Group A Streptococcus, a Gram-positive human-specific pathogen that causes pharyngitis, impetigo, invasive disease, necrotizing fasciitis, and autoimmune sequelae has long, surface-exposed, pilus-like structures composed of members of a family of extracellular matrix-binding proteins.
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